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checkmate vectors pbind  (Promega)

 
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    Promega checkmate vectors pbind
    Checkmate Vectors Pbind, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/checkmate vectors pbind/product/Promega
    Average 90 stars, based on 1 article reviews
    checkmate vectors pbind - by Bioz Stars, 2026-05
    90/100 stars

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    Inhibition of TRAP230 function in transfected COS-7 cells by the TRAP230-derived M40 fragment. Increasing amounts of the TRAP230/M40 fragment fused to HA tag (HA-M40) inhibit the transactivation capacity <t>of</t> <t>GAL4-SOX9</t> TA-domain, whereas HA-M40 has no inhibitory effect on the transactivation capacity of GAL4-VP16. COS-7 cells were transfected with 10 ng of <t>pBIND</t> SOX9-TA-domain or pBIND VP16-TA-domain, 500 ng of GAL4-responsive luciferase reporter plasmid (5× UAS Luc) and either 10 or 20 ng of pcDNA HA-M40, or an empty vector pcDNA (20 ng). Luciferase values have been normalized to SOX9-TA mediated activation without pcDNA Ha-M40 but with an empty pcDNA taken as 100%, which corresponds to a 10–15-fold induction from one experiment to another. Results are expressed as percentage inhibition of the SOX9 TA-domain activity and represent the means of triplicate experiments performed six times.
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    Inhibition of TRAP230 function in transfected COS-7 cells by the TRAP230-derived M40 fragment. Increasing amounts of the TRAP230/M40 fragment fused to HA tag (HA-M40) inhibit the transactivation capacity of GAL4-SOX9 TA-domain, whereas HA-M40 has no inhibitory effect on the transactivation capacity of GAL4-VP16. COS-7 cells were transfected with 10 ng of pBIND SOX9-TA-domain or pBIND VP16-TA-domain, 500 ng of GAL4-responsive luciferase reporter plasmid (5× UAS Luc) and either 10 or 20 ng of pcDNA HA-M40, or an empty vector pcDNA (20 ng). Luciferase values have been normalized to SOX9-TA mediated activation without pcDNA Ha-M40 but with an empty pcDNA taken as 100%, which corresponds to a 10–15-fold induction from one experiment to another. Results are expressed as percentage inhibition of the SOX9 TA-domain activity and represent the means of triplicate experiments performed six times.

    Journal:

    Article Title: SOX9 interacts with a component of the human thyroid hormone receptor-associated protein complex

    doi:

    Figure Lengend Snippet: Inhibition of TRAP230 function in transfected COS-7 cells by the TRAP230-derived M40 fragment. Increasing amounts of the TRAP230/M40 fragment fused to HA tag (HA-M40) inhibit the transactivation capacity of GAL4-SOX9 TA-domain, whereas HA-M40 has no inhibitory effect on the transactivation capacity of GAL4-VP16. COS-7 cells were transfected with 10 ng of pBIND SOX9-TA-domain or pBIND VP16-TA-domain, 500 ng of GAL4-responsive luciferase reporter plasmid (5× UAS Luc) and either 10 or 20 ng of pcDNA HA-M40, or an empty vector pcDNA (20 ng). Luciferase values have been normalized to SOX9-TA mediated activation without pcDNA Ha-M40 but with an empty pcDNA taken as 100%, which corresponds to a 10–15-fold induction from one experiment to another. Results are expressed as percentage inhibition of the SOX9 TA-domain activity and represent the means of triplicate experiments performed six times.

    Article Snippet: The SOX9 TA-domain (amino acids 408–509) was cloned into pBIND vector (Checkmate two-hybrid System; Promega), thus creating a fusion construct encoding both the yeast GAL4 DNA-binding domain and the SOX9 TA-domain.

    Techniques: Inhibition, Transfection, Derivative Assay, Luciferase, Plasmid Preparation, Activation Assay, Activity Assay